Goat anti-Cow IgG (Whole Molecule) Antibody (Biotin) - Preadsorbed
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- Target See all IgG products
- IgG
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Binding Specificity
- Whole Molecule
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Reactivity
- Cow
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Biotin
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Application
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Characteristics
- Molar Ratio: 10-20 BAC molecules per Goat IgG molecule.
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
- Bovine IgG whole molecule.
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- Application Notes
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Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. This product has been assayed against 1.0 g of Bovine IgG in a standardcapture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1:11,000 to 1:45,000 of the reconstitution concentration is suggestedfor this product.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore with 1.0 mL of deionized water (or equivalent).
- Concentration
- 2.0 mg/mL
- Buffer
- 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 contaning 0.01 % (w/v) Thimerisol as preservative and 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- Biohazard Informations: This product contains thimerosal which is hazardous.
- Handling Advice
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Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Synonyms: Bovine Immunoglobulin G
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