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Rabbit anti-Human IgG3 Antibody (FITC)

WB, IHC, IF, ICC Polyclonal IgG FITC
Catalog No. ABIN1173722
  • Target See all IgG3 products
    IgG3
    Reactivity
    • 78
    • 18
    • 3
    Human
    Host
    • 37
    • 34
    • 17
    • 10
    • 1
    Rabbit
    Clonality
    • 64
    • 26
    Polyclonal
    Conjugate
    • 19
    • 12
    • 12
    • 11
    • 7
    • 5
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    FITC
    Application
    • 57
    • 43
    • 39
    • 32
    • 29
    • 21
    • 10
    • 9
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    It has been selected for its ability to recognize IgG3 in immunohistochemical staining and Western blotting.
    Purification
    Affinity Chromatography
    Immunogen
    The antibody is a rabbit polyclonal antibody raised against IgG3 conjugated to fitc.
    Isotype
    IgG
  • Application Notes
    Western blotting: 1:100-400
    Immunocytochemistry in formalin fixed cells: 1:100-500
    Immunohistochemistry in formalin fixed frozen section: 1:100-500
    Immunohistochemistry in paraffin section: 1:50-200
    Enzyme-linked Immunosorbent Assay: 1:100-200
    Optimal working dilutions must be determined by end user.
    Comment

    Excitation/Emission wavelength: 494 nm/514 nm

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    200 µg/mL
    Buffer
    Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freeze/thaw cycles
    Storage
    4 °C
    Storage Comment
    Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
    Expiry Date
    12 months
  • Target
    IgG3
    Abstract
    IgG3 Products
    Target Type
    Antibody
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