Goat anti-Mouse IgG1 (Heavy Chain) Antibody (TRITC) - Preadsorbed
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- Target See all IgG1 products
- IgG1
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Binding Specificity
- Heavy Chain
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Reactivity
- Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- TRITC
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), FLISA, Fluorescence Microscopy (FM)
- Specificity
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Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.
Specificity was confirmed by ELISA at less than 0.5 % of target signal. - Cross-Reactivity
- Mouse (Murine)
- Characteristics
- Rhodamine Conjugated Secondary Antibodies are ideal for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting and are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Mouse IgG1 heavy chain
Immunogen Type: Native Protein
- Isotype
- IgG1
- Labeling Ratio
- 3.23
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- Application Notes
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Mouse IgG1 Antibody Rhodamine Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
IF Microscopy Dilution: 1:1,000 - 1:5,000
- Comment
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Rhodamine Conjugated Secondary Antibodies are ideal for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting and are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light.
Centrifuge product if not completely clear after standing at room temperature. - Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
- Expiry Date
- 12 months
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- Target
- IgG1
- Abstract
- IgG1 Products
- Target Type
- Antibody
- Background
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Synonyms: Goat anti-mouse IgG1 antibody rhodamine conjugation, goat anti-mouse IgG1 (gamma 1) TRITC conjugated antibody
Background: Anti-Mouse IgG1 Rhodamine Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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