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Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody - Preadsorbed

ELISA, IHC, WB Polyclonal IgG unconjugated F(ab')2 fragment
Catalog No. ABIN1044055
  • Target See all IgG products
    IgG
    Fragment
    F(ab')2 fragment
    Binding Specificity
    • 2382
    • 512
    • 424
    • 207
    • 132
    • 129
    • 95
    • 29
    • 28
    • 28
    • 22
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    Heavy & Light Chain
    Reactivity
    • 724
    • 699
    • 601
    • 477
    • 403
    • 243
    • 212
    • 176
    • 147
    • 138
    • 113
    • 112
    • 107
    • 102
    • 82
    • 59
    • 47
    • 28
    • 28
    • 16
    • 12
    • 10
    • 8
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Rabbit
    Host
    • 1778
    • 1576
    • 571
    • 241
    • 112
    • 111
    • 33
    • 31
    • 21
    • 19
    • 15
    • 11
    • 11
    • 1
    Donkey
    Clonality
    • 3434
    • 175
    • 1
    Polyclonal
    Application
    • 1952
    • 1838
    • 1806
    • 1519
    • 934
    • 910
    • 652
    • 478
    • 380
    • 236
    • 236
    • 234
    • 106
    • 103
    • 86
    • 67
    • 53
    • 52
    • 31
    • 28
    • 25
    • 21
    • 18
    • 16
    • 11
    • 9
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Rabbit IgG, and Rabbit Serum.
    Purification
    Preadsorption: Solid phase absorption
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Rabbit IgG whole molecule

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:1,000-1:5,000

    Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

    ELISA Dilution: 1:20,000-1:100,000

    Western Blot Dilution: 1:2,000-1:10,000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4 °C prior to opening. This product is stable for several weeks at 4 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -24 °C or below.
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background

    Synonyms: Donkey F(ab')2 Anti-Rabbit IgG Pre-Adsorbed Antibody, Donkey Fab2 Anti-Rabbit IgG Antibody

    Background: F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

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