Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (TRITC) - Preadsorbed

Catalog No. ABIN1044054

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Donkey

Clonality: Polyclonal

Conjugate: TRITC

Application: Fluorescence Microscopy (FM)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Rabbit IgG and Rabbit Serum.

Characteristics: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Rabbit IgG whole molecule

Isotype: IgG

Labeling Ratio: 2.5

Application Details

Application Notes:

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

IF Microscopy Dilution: 1:500-1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Product is photosensitive and should be protected from light.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4 °C prior to opening. This product is stable for several weeks at 4 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -24 °C or below.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Donkey F(ab')2 Anti-Rabbit IgG Rhodamine Conjugated Antibody, Donkey Fab2 Anti-Rabbit IgG Antibody TRITC Conjugation

Background: F(ab')2 Anti-Rabbit IgG (H&L) Antibody generated in donkey detects rabbit IgG. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This F(ab')2 Anti-Rabbit IgG Antibody is conjugated to Rhodamine.