Donkey anti-Chicken IgG (Heavy & Light Chain) Antibody (TRITC) - Preadsorbed

Catalog No. ABIN1043928

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Chicken

Host: Donkey

Clonality: Polyclonal

Conjugate: TRITC

Application: FLISA, Fluorescence Microscopy (FM)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Chicken IgG and Chicken Serum.

Cross-Reactivity: Chicken

Characteristics: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purification: Preadsorption: immunoaffinity chromatography using Chicken IgG coupled to agarose beads

Immunogen:

Immunogen: Chicken IgG whole molecule

Isotype: IgG

Labeling Ratio: 3.1

Application Details

Application Notes:

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

IF Microscopy Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light.
Centrifuge product if not completely clear after standing at room temperature.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Donkey anti-Chicken IgG Antibody Rhodamine Conjugation, Donkey anti-Chicken IgG TRITC Conjugated Antibody

Background: Anti-Chicken IgG Antibody generated in donkey detects chicken IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.