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Rabbit anti-Rat IgM Antibody (HRP)

Rockland 212-4307 ELISA, IHC, WB Polyclonal IgG HRP
Rockland
Catalog No. ABIN102716
Supplier Product No.: 212-4307
  • Target See all IgM products
    IgM
    Reactivity
    • 224
    • 159
    • 90
    • 42
    • 34
    • 34
    • 29
    • 24
    • 17
    • 14
    • 13
    • 13
    • 13
    • 13
    • 12
    • 4
    • 2
    Rat
    Host
    • 373
    • 230
    • 84
    • 19
    • 14
    • 5
    • 4
    • 1
    • 1
    Rabbit
    Clonality
    • 539
    • 90
    Polyclonal
    Conjugate
    • 121
    • 86
    • 85
    • 66
    • 34
    • 32
    • 26
    • 24
    • 14
    • 12
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 7
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    HRP
    Application
    • 383
    • 288
    • 282
    • 242
    • 182
    • 137
    • 91
    • 91
    • 64
    • 62
    • 54
    • 53
    • 45
    • 30
    • 18
    • 17
    • 10
    • 6
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Supplier Product No.
    212-4307
    Supplier
    Rockland
    Purpose
    Rat IgM (mu) Antibody Peroxidase Conjugated
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgM and Rat Serum.  No reaction was observed against Rat IgG.
    Characteristics
    Anti-Rat IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the rat IgG molecule. Rat IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind rat IgG F(c) will not bind rat IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rat IgG F(ab')2 is conjugated to Peroxidase.
    Purification
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by cross adsorption to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
    Immunogen
    Optional[Immunogen]: Rat IgM mu heavy chain
    Isotype
    IgG
  • Application Notes
    Application Note: This product has been assayed against 1.0 μg of Rat IgM in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3,500 to 1:15,000 of the reconstitution concentration is suggested for this product.  Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
    Concentration
    10.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    IgM
    Abstract
    IgM Products
    Target Type
    Antibody
    Background
    Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Rat IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology. This Anti-Rat IgM is conjugated to Peroxidase.
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