Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Rabbit anti-Rat IgM Antibody (FITC)

Rockland 212-4207 FACS, FLISA, FM Polyclonal IgG FITC
Rockland
Catalog No. ABIN102714
Supplier Product No.: 212-4207
  • Target See all IgM products
    IgM
    Reactivity
    • 224
    • 159
    • 90
    • 42
    • 34
    • 34
    • 29
    • 24
    • 17
    • 14
    • 13
    • 13
    • 13
    • 13
    • 12
    • 4
    • 2
    Rat
    Host
    • 373
    • 230
    • 84
    • 19
    • 14
    • 5
    • 4
    • 1
    • 1
    Rabbit
    Clonality
    • 539
    • 90
    Polyclonal
    Conjugate
    • 121
    • 86
    • 85
    • 66
    • 34
    • 32
    • 26
    • 24
    • 14
    • 12
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 7
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    FITC
    Application
    • 384
    • 287
    • 283
    • 243
    • 182
    • 137
    • 91
    • 91
    • 63
    • 61
    • 54
    • 53
    • 45
    • 30
    • 18
    • 17
    • 10
    • 6
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Supplier Product No.
    212-4207
    Supplier
    Rockland
    Purpose
    Rat IgM (mu) Antibody Fluorescein Conjugated
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Rat IgM and Rat Serum.  No reaction was observed against Rat IgG.
    Characteristics
    Anti-Rat IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the rat IgG molecule. Rat IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind rat IgG F(c) will not bind rat IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rat IgG F(ab')2 is conjugated to Fluorescein.
    Purification
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    Immunogen
    Optional[Immunogen]: Rat IgM mu heavy chain
    Isotype
    IgG
  • Application Notes
    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
    Concentration
    10.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    IgM
    Abstract
    IgM Products
    Target Type
    Antibody
    Background
    Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Rat IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology. This Anti-Rat IgM antibody is conjugated to fluorescein.
You are here:
Support