Rabbit anti-Rat IgM (Heavy Chain) Antibody

Catalog No. ABIN102709

Product Details

Target: IgM

Binding Specificity: Heavy Chain

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgM µ chain

Characteristics: Concentration Definition: by Refractometry

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Rat IgM and Rat Serum.  No reaction was observed against Rat IgG F(ab')2  or Rat IgG F(c).

Immunogen:

Immunogen: Rat IgM mu heavy chain

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Rat IgM antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Expiry Date: 12 months

References

Regal, Strehlke, Peterson, Wing, Parker, Nieto, Bemis, Gilbert, Fleming: "Role of IgM and angiotensin II Type I receptor autoantibodies in local complement activation in placental ischemia-induced hypertension in the rat." in: Molecular immunology, Vol. 78, pp. 38-47, (2017) (PubMed).

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody

Background:

Synonyms: rabbit anti-Rat IgM (mu) Antibody, rabbit anti Rat IgM mu

Background: Anti-Rat IgM antibody generated in rabbit specifically detects rat IgM mu heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Rat IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.