Goat anti-Rat IgM Antibody

Catalog No. ABIN102702

Supplier Product No.: 612-1107

Product Details

Target: IgM

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.: 612-1107

Supplier: Rockland

Purpose: Rat IgM (mu chain) Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgM and Rat Serum.  No reaction was observed against other rat heavy or light chain proteins.

Characteristics: Rat IgA antibodies detects the alpha chain of the rat IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA and Rat Serum. No reaction was observed against Rat IgM or Rat IgG. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other Rat heavy or light chain isotypes. Anti-Rat IgA antibody is ideal for investigators involved in serum component research.

Purification: Anti-RAT IgM (mu chain) (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Rat IgM whole molecule

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-RAT IgM (mu chain) (GOAT) Antibody has been tested by ELISA and western blot and is assayed against Rat IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin. A working dilution of 1:15,000 to 1:70,000 of the concentration is suggested for this product. Rat IgM antibody is suitable for immunoassays including western blot and ELISA. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:1,000 to 1:10,000 ELISA Dilution: 1:80,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.16 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody

Background: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Rat IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.