Goat anti-Rabbit IgM (Heavy Chain) Antibody

Catalog No. ABIN102693

Product Details

Target: IgM

Binding Specificity: Heavy Chain

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgM µ chain

Characteristics: Concentration Definition: by Refractometry

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Rabbit IgM and Rabbit Serum.  No reaction was observed against Rabbit IgG.

Immunogen:

Immunogen: Rabbit IgM mu heavy chain

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Rabbit IgM antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Comment:

Protein Concentration: 90.0 mg/mL (by Refractometry)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Expiry Date: 12 months

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody

Background:

Synonyms: goat anti-Rabbit IgM (mu) Antibody, goat anti Rabbit IgM mu

Background: Anti-Rabbit IgM antibody generated in goat specifically detects rabbit IgM mu heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Rabbit IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.