Goat anti-Mouse IgM Antibody (FITC)

Catalog No. ABIN102677

Supplier Product No.: 610-1207

Product Details

Target: IgM

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: FITC

Application: Flow Cytometry (FACS), Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Supplier Product No.: 610-1207

Supplier: Rockland

Purpose: Mouse IgM (mu chain) Antibody Fluorescein Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against other mouse heavy or light chain proteins.

Characteristics: Anti-Mouse IgG F(ab')2 Fluorescein Antibody generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind mouse IgG F(c) will not bind mouse IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Mouse IgM whole molecule

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Mouse IgM antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody

Background: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Mouse IgM Fluorescein antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.