Goat anti-Mouse IgM (Heavy & Light Chain) Antibody - Preadsorbed

Catalog No. ABIN102674

Product Details

Target: IgM

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgM µ chain

Cross-Reactivity: Mouse (Murine)

Characteristics: Anti-Mouse IgM (mu chain) antibody generated in goat detects specifically mouse IgM (mu chain). This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Sterility: Sterile filtered

Immunogen:

Immunogen: Mouse IgM whole molecule

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Mouse IgM (mu chain) is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.

ELISA Dilution: 1:20,400 - 1:40,400

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody

Background:

Synonyms: goat anti-Mouse IgM (mu chain) Antibody, Gt-a-Mouse (mu chain) antibody, Mouse IgM (mu chain) Antibody in goat, Goat anti-mouse IgM Secondary Antibody

Background: Anti-Mouse IgM (mu chain) antibody generated in goat detects specifically mouse IgM (mu chain). Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Mouse IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.