Rabbit anti-Human IgM (Fc5mu Region) Antibody - Preadsorbed

Catalog No. ABIN102640

Product Details

Target: IgM

Binding Specificity: Fc5mu Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgM Fc5µ

Characteristics: Anti-Human IgM Fc5µ antibody generated in rabbit detects specifically human IgM. This anti-human IgM secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Human IgM Fc5mu was produced by repeated immunization with Human IgM Fc5mu fragment in rabbit.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:3,000

Application Note: Human IgM Fc5μ antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

ELISA Dilution: 1:20,000 - 1:50,000

Western Blot Dilution: 1:500 - 1:3,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody

Background:

Synonyms: rabbit anti-Human IgM Fc5μ Antibody, Human IgM Fc5u Antibody, Rb-a-Human IgM Fc5μ Antibody, Human IgM Fc5μ Antibody in Rabbit, Human M Fc5μ Secondary Antibody

Background: Anti-Human IgM Fc5μ antibody generated in rabbit specifically detects Fc5μ portion of the human IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Due to this large size, IgM is typically isolated to the serum. Anti-Human IgM Fc5μ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.