Goat anti-Human IgM (Heavy & Light Chain) Antibody - Preadsorbed

Catalog No. ABIN102624

Product Details

Target: IgM

Binding Specificity: Heavy & Light Chain

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgM µ chain

Cross-Reactivity: Human

Characteristics: Anti-Mouse IgG3 heavy chain antibody generated in rabbit detects specifically Mouse IgG3 heavy chain. This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Sterility: Sterile filtered

Immunogen:

Immunogen: Human IgM whole molecule

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Human IgM mu heavy chain is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

ELISA Dilution: 1:10,000 - 1:50,000

Western Blot Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

References

Yang, Jacobs, McCallen, Moore, Huckaby, Edelstein, Lai: "Analysis of Pre-existing IgG and IgM Antibodies against Polyethylene Glycol (PEG) in the General Population." in: Analytical chemistry, Vol. 88, Issue 23, pp. 11804-11812, (2018) (PubMed).

Target Details

Target: IgM

Alternative Name: IgM mu (IgM Products)

Target Type: Antibody

Background:

Synonyms: goat anti-Human IgM mu chain Antibody, Gt-a-Human IgM, Human IgM Antibody in Goat, Human Secondary Antibody

Background: Anti-Human IgM (mu heavy chain) generated in goat detects specifically Human IgM mu heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Anti-Human IgM mu chain antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.