Rabbit anti-Cow IgM (Heavy Chain) Antibody (FITC)
-
- Target See all IgM products
- IgM
-
Binding Specificity
- Heavy Chain
-
Reactivity
- Cow
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- FITC
-
Application
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Specificity
- IgM µ chain
- Characteristics
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Bovine IgM and Bovine Serum. No reaction was observed against Bovine IgG.
- Immunogen
-
Immunogen: Bovine IgM mu heavy chain
- Isotype
- IgG
- Labeling Ratio
- 5.2
-
-
- Application Notes
-
Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:1:5,000
- Comment
-
Excitation/Emission wavelength: 494 nm/514 nm
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 10.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Product is photosensitive and should be protected from light.
- Storage
- RT,4 °C,-20 °C
- Expiry Date
- 12 months
-
- Target
- IgM
- Abstract
- IgM Products
- Target Type
- Antibody
- Background
-
Synonyms: rabbit anti-Bovine IgM mu chain Antibody Fluorescein Conjugated, rabbit anti-Bovine IgM mu chain FITC Conjugated Antibody
Background: Immunoglobulin M is the largest antibody isotype and the first to be secrected against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approixmate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Bovine IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
-
