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Rabbit anti-Mouse IgG2a (Heavy Chain) Antibody (FITC) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG FITC
Catalog No. ABIN102376
  • Target See all IgG2a products
    IgG2a
    Binding Specificity
    • 50
    • 15
    • 7
    • 2
    • 2
    • 2
    • 1
    • 1
    Heavy Chain
    Reactivity
    • 97
    • 18
    • 1
    Mouse
    Host
    • 57
    • 28
    • 13
    • 13
    • 5
    Rabbit
    Clonality
    • 71
    • 26
    Polyclonal
    Conjugate
    • 22
    • 13
    • 12
    • 10
    • 9
    • 5
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    FITC
    Application
    • 58
    • 45
    • 38
    • 33
    • 23
    • 18
    • 11
    • 10
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Specificity
    IgG2a
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: immunoaffinity chromatography using Mouse IgG2a coupled to agarose beads
    Immunogen

    Immunogen: Mouse IgG2a heavy chain

    Isotype
    IgG
    Labeling Ratio
    3.7
  • Application Notes

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Comment

    Excitation/Emission wavelength: 494 nm/514 nm

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Product is photosensitive and should be protected from light.
    Storage
    RT,4 °C,-20 °C
    Expiry Date
    12 months
  • Target
    IgG2a
    Abstract
    IgG2a Products
    Target Type
    Antibody
    Background

    Synonyms: Rabbit Anti-Mouse IgG2a (gamma 2a chain) Antibody fluorescein Conjugated, Rabbit Anti-Mouse IgG2a Antibody FITC Conjugation

    Background: Anti-Mouse IgG2a Fluorescein Antibody generated in rabbit detects reactivity to Mouse IgG2a (Gamma 2a chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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