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Rabbit anti-Mouse IgG1 (Heavy Chain) Antibody (Alkaline Phosphatase (AP))

ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)
Catalog No. ABIN102334
  • Target See all IgG1 products
    IgG1
    Binding Specificity
    • 51
    • 26
    • 17
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Heavy Chain
    Reactivity
    • 95
    • 24
    • 23
    • 7
    • 7
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    Mouse
    Host
    • 63
    • 41
    • 37
    • 14
    • 10
    • 6
    • 1
    Rabbit
    Clonality
    • 94
    • 57
    Polyclonal
    Conjugate
    • 46
    • 20
    • 19
    • 19
    • 10
    • 8
    • 7
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Alkaline Phosphatase (AP)
    Application
    • 84
    • 58
    • 53
    • 45
    • 32
    • 21
    • 18
    • 15
    • 11
    • 10
    • 10
    • 9
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
    Specificity
    IgG1
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum.  Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Mouse IgG1 heavy chain

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:200 - 1:1,000

    Application Note: Mouse IgG1 secondary antibody conjugated to Alkaline Phosphatase is available in a variety of formats. Anti Mouse IgG1 secondary antibody conjugate is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.

    ELISA Dilution: 1:5,000

    Western Blot Dilution: 1:500 - 1:2,500

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Do not add Sodium azide.
    Dilute only prior to immediate use
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Storage
    4 °C
    Expiry Date
    12 months
  • Target
    IgG1
    Abstract
    IgG1 Products
    Target Type
    Antibody
    Background

    Synonyms: Rabbit Anti-Mouse IgG1 (Gamma 1 chain) Antibody alkaline phosphatase Conjugation, Rabbit Anti-Mouse IgG1 alk phos Conjugated Antibody

    Background: Anti-Mouse IgG1 Alkaline Phosphatase Antibody generated in rabbit detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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