Goat anti-Rat IgG (Fc Region) Antibody (Biotin) - Preadsorbed
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- Target See all IgG products
- IgG
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Binding Specificity
- Fc Region
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Reactivity
- Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Biotin
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- IgG F(c)
- Characteristics
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- Preadsorption: immunoaffinity chromatography using Rat IgG coupled to agarose beads
- Immunogen
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Immunogen: Rat IgG F(c) fragment
- Isotype
- IgG
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Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 488) - Preadsorbed
WB, FLISA, FM Polyclonal IgG Atto 488
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 647N) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 647N
Goat anti-Rat IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Goat anti-Rat IgG (Heavy & Light Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 425) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 425
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 532) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 532
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 594) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 594
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin
Goat anti-Rat IgG (Heavy Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
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- Application Notes
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: This product has been assayed against 1.0 μg of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 2.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Aliquot to Avoid repeated freezing and thawing.
- Storage
- RT,4 °C,-20 °C
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
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Synonyms: Goat Anti Rat IgG F(c) Biotin Conjugated Antibody, Goat Anti-Rat IgG Fc Fragment Biotin Conjugated Antibody, Goat Anti-Rat IgG Fc Antibody Biotin Conjugation
Background: Anti-Rat IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of rat IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
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