Goat anti-Rabbit IgG (Fc Region) Antibody - Preadsorbed

Catalog No. ABIN102079

Supplier Product No.: 611-1123

Product Details

Target: IgG

Binding Specificity: Fc Region

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Supplier Product No.: 611-1123

Supplier: Rockland

Purpose: Rabbit IgG Fc Antibody Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab) or Human Serum Proteins.

Characteristics: Anti-Rabbit IgG (H&L) Antibody generated in goat detects reactivity to Rabbit IgG.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti Rabbit IgG F(c) was produced by repeated immunization with rabbit IgG F(c) fragment in goat.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Rabbit IgG F(c) is tested by immunoelectrophoresis, ELISA, dot blot, and western blot. This product is suitable for use in western-blot, competitive western-blot, and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Expect ~25 kDa for Rabbit IgG F(c) Fragment. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.