Sheep anti-Rabbit IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN102021

Supplier Product No.: 611-6502

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Sheep

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Supplier Product No.: 611-6502

Supplier: Rockland

Purpose: Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Sheep Serum, Rabbit IgG and Rabbit Serum.

Characteristics: Anti-Rabbit IgG peroxidase conjugated antibody generated in sheep detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Purification: Anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Rabbit IgG whole molecule

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Rabbit IgG Alkaline Phosphatase antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunohistochemistry applications. Specific conditions for reactivity should be optimized by the researcher. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:15,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Rabbit IgG antibody detects rabbit IgG whole molecule heavy and light chain components. Anti-Rabbit IgG antibody is ideal for researchers using immunoassays with rabbit primary antibody reagents.