Sheep anti-Rabbit IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))
-
- Target See all IgG products
- IgG
-
Binding Specificity
- Heavy & Light Chain
-
Reactivity
- Rabbit
-
Host
- Sheep
-
Clonality
- Polyclonal
-
Conjugate
- Alkaline Phosphatase (AP)
-
Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
- Supplier Product No.
- 611-6502
- Supplier
- Rockland
- Purpose
- Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Sheep Serum, Rabbit IgG and Rabbit Serum.
- Characteristics
- Anti-Rabbit IgG peroxidase conjugated antibody generated in sheep detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
- Purification
- Anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- Sterility
- Sterile filtered
- Immunogen
- Optional[Immunogen]: Rabbit IgG whole molecule
- Isotype
- IgG
-
Guinea Pig anti-Rabbit IgG (Heavy & Light Chain) antibody - Preadsorbed
ELISA, IHC, WB, CUT&RUN, CUT&Tag Polyclonal IgG unconjugated
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (FITC)WB, FACS, FLISA, FM, DB Polyclonal IgG FITC
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)High-quality product from Rockland ELISA, IHC, WB, DB Polyclonal IgG HRP
Goat anti-Rabbit IgG antibody (DyLight 800)ELISA, WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rabbit IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Goat anti-Rabbit IgG antibody (DyLight 488)WB, IF, FLISA, FM, DB, MA Polyclonal IgG DyLight 488
Goat anti-Rabbit IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
-
- Application Notes
- Application Note: Anti-Rabbit IgG Alkaline Phosphatase antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunohistochemistry applications. Specific conditions for reactivity should be optimized by the researcher. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:15,000 Other: User Optimized
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
-
Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- Expiry Date
- 12 months
-
- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Rabbit IgG antibody detects rabbit IgG whole molecule heavy and light chain components. Anti-Rabbit IgG antibody is ideal for researchers using immunoassays with rabbit primary antibody reagents.
-
