Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN102010

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: Immunoassay (IA)

Specificity: IgG (H&L)

Characteristics: Anti Rabbit HRP Conjugated Secondary Antibodies are ideal for Western Blotting, Immunohistochemistry, ELISA as well as other anibody detection methods.
Concentration Definition: by UV absorbance at 280 nm

Immunogen: Rabbit IgG whole molecule

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Gentamicin sulfate

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

References

Putta, Smith, Chaudhuri, Guardia-Wolff, Rosenbaum, Graham: "Activation of the cytosolic calcium-independent phospholipase A2 β isoform contributes to TRPC6 externalization via release of arachidonic acid." in: The Journal of biological chemistry, Vol. 297, Issue 4, pp. 101180, (2021) (PubMed).

Grundmann, Merten, Malfacini, Inoue, Preis, Simon, Rüttiger, Ziegler, Benkel, Schmitt, Ishida, Müller, Reher, Kawakami, Inoue, Rick, Kühl, Imhof, Aoki, König, Hoffmann, Gomeza, Wess, Kostenis: "Lack of beta-arrestin signaling in the absence of active G proteins." in: Nature communications, Vol. 9, Issue 1, pp. 341, (2018) (PubMed).

Bartlett, Sluyter, Watson, Sluyter, Yerbury: "P2X7 antagonism using Brilliant Blue G reduces body weight loss and prolongs survival in female SOD1G93A amyotrophic lateral sclerosis mice." in: PeerJ, Vol. 5, pp. e3064, (2017) (PubMed).

Lampi, Faber, Huynh, Bordeleau, Zanotelli, Reinhart-King: "Simvastatin Ameliorates Matrix Stiffness-Mediated Endothelial Monolayer Disruption." in: PLoS ONE, Vol. 11, Issue 1, pp. e0147033, (2016) (PubMed).

Dickerman, White, Kessler, Sadler, Williams, Sen: "The protein activator of protein kinase R, PACT/RAX, negatively regulates protein kinase R during mouse anterior pituitary development." in: The FEBS journal, Vol. 282, Issue 24, pp. 4766-81, (2016) (PubMed).

DLima, Ma, Winkler, Chu, Loh, Corpuz, Budnik, Lykke-Andersen, Saghatelian, Slavoff: "A human microprotein that interacts with the mRNA decapping complex." in: Nature chemical biology, Vol. 13, Issue 2, pp. 174-180, (2016) (PubMed).

Schmitz, Schrage, Gaffal, Charpentier, Wiest, Hiltensperger, Morschel, Hennen, Häußler, Horn, Wenzel, Grundmann, Büllesbach, Schröder, Brewitz, Schmidt, Gomeza, Galés, Fleischmann, Tüting, Imhof et al.: "A cell-permeable inhibitor to trap Gαq proteins in the empty pocket conformation. ..." in: Chemistry & biology, Vol. 21, Issue 7, pp. 890-902, (2015) (PubMed).

Dickerman, McDonald, Sen: "The Human dsRNA binding protein PACT is unable to functionally substitute for the Drosophila dsRNA binding protein R2D2." in: F1000Research, Vol. 2, pp. 220, (2014) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Synonyms: goat anti rabbit conjugated secondary antibody