Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN102010

Supplier Product No.: 611-103-122

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Supplier Product No.: 611-103-122

Supplier: Rockland

Purpose: Rabbit IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

Characteristics: Anti-Rabbit IgG F(ab')2 Antibody generated in goat recognizes the dimeric Fab portion of the rabbit IgG molecule. Rabbit IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Rabbit IgG F(c) will not bind rabbit IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG F(ab')2 Antibody is conjugated to Rhodamine.

Purification: Conjugated Second Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Rabbit IgG whole molecule

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Rabbit secondary antibody conjugated to horseradish peroxidase (HRP) generated in goat detects specifically rabbit IgG. This anti-rabbit HRP mx10 secondary antibody has been tested in ELISA, Dot Blot, and Western Blot. Although not tested, this product could be useful for Sandwich ELISA, titration assays, immunoprecipitation, immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store secondary antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References

Putta, Smith, Chaudhuri, Guardia-Wolff, Rosenbaum, Graham: "Activation of the cytosolic calcium-independent phospholipase A2 β isoform contributes to TRPC6 externalization via release of arachidonic acid." in: The Journal of biological chemistry, Vol. 297, Issue 4, pp. 101180, (2021) (PubMed).

Grundmann, Merten, Malfacini, Inoue, Preis, Simon, Rüttiger, Ziegler, Benkel, Schmitt, Ishida, Müller, Reher, Kawakami, Inoue, Rick, Kühl, Imhof, Aoki, König, Hoffmann, Gomeza, Wess, Kostenis: "Lack of beta-arrestin signaling in the absence of active G proteins." in: Nature communications, Vol. 9, Issue 1, pp. 341, (2018) (PubMed).

Bartlett, Sluyter, Watson, Sluyter, Yerbury: "P2X7 antagonism using Brilliant Blue G reduces body weight loss and prolongs survival in female SOD1G93A amyotrophic lateral sclerosis mice." in: PeerJ, Vol. 5, pp. e3064, (2017) (PubMed).

Lampi, Faber, Huynh, Bordeleau, Zanotelli, Reinhart-King: "Simvastatin Ameliorates Matrix Stiffness-Mediated Endothelial Monolayer Disruption." in: PLoS ONE, Vol. 11, Issue 1, pp. e0147033, (2016) (PubMed).

Dickerman, White, Kessler, Sadler, Williams, Sen: "The protein activator of protein kinase R, PACT/RAX, negatively regulates protein kinase R during mouse anterior pituitary development." in: The FEBS journal, Vol. 282, Issue 24, pp. 4766-81, (2016) (PubMed).

DLima, Ma, Winkler, Chu, Loh, Corpuz, Budnik, Lykke-Andersen, Saghatelian, Slavoff: "A human microprotein that interacts with the mRNA decapping complex." in: Nature chemical biology, Vol. 13, Issue 2, pp. 174-180, (2016) (PubMed).

Schmitz, Schrage, Gaffal, Charpentier, Wiest, Hiltensperger, Morschel, Hennen, Häußler, Horn, Wenzel, Grundmann, Büllesbach, Schröder, Brewitz, Schmidt, Gomeza, Galés, Fleischmann, Tüting, Imhof et al.: "A cell-permeable inhibitor to trap Gαq proteins in the empty pocket conformation. ..." in: Chemistry & biology, Vol. 21, Issue 7, pp. 890-902, (2015) (PubMed).

Dickerman, McDonald, Sen: "The Human dsRNA binding protein PACT is unable to functionally substitute for the Drosophila dsRNA binding protein R2D2." in: F1000Research, Vol. 2, pp. 220, (2014) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Goat anti-Rabbit IgG (H&L) Antibody Conjugated to Horseradish Peroxidase (HRP) is designed for chemiluminescent detection. Secondary antibodies bind to the primary antibody to assist in detection, sorting and purification of target antigens. To enable detection, the secondary antibody must have specificity for the antibody species and isotype of the primary antibody being used and generally is conjugated. This purified secondary antibody has been validated and optimized yielding good sensitivity and reproducible results with Rockland's primary antibodies. This anti-rabbit IgG antibody has been crossed adsorbed to show no reaction against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.