Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody

Catalog No. ABIN101983

Supplier Product No.: 611-1102

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Fluorescence Microscopy (FM), Immunoprecipitation (IP), Dot Blot (DB)

Supplier Product No.: 611-1102

Supplier: Rockland

Purpose: Rabbit IgG (H&L) Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.

Characteristics: Anti-Rabbit IgG Antibody Texas Red™ generated in goat detects rabbit IgG.

Purification: Rabbit Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in goat.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Rabbit IgG (H&L) has been tested by ELISA, dot blot, and western blot. This antibody is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 Immunoprecipitation Dilution: User Optimized ELISA Dilution: 1:10,000 - 1:60,000 IF Microscopy Dilution: User Optimized Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.2 mg/mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Rabbit Secondary Antibody at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References

Li, Wu, Yang, Liu, Chen: "Sesquiterpene lactone 6-O-angeloylplenolin reverses vincristine resistance by inhibiting YB-1 nuclear translocation in colon carcinoma cells." in: Oncology letters, Vol. 15, Issue 6, pp. 9673-9680, (2018) (PubMed).

Skytt, Toft-Kehler, Brændstrup, Cejvanovic, Gurubaran, Bergersen, Kolko: "Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Müller Cells and Retinal Ganglion Cells." in: BioMed research international, Vol. 2016, pp. 1087647, (2017) (PubMed).

Stephen, Higgs, Sheehan, Al Awabdh, López-Doménech, Arancibia-Carcamo, Kittler: "Miro1 Regulates Activity-Driven Positioning of Mitochondria within Astrocytic Processes Apposed to Synapses to Regulate Intracellular Calcium Signaling." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 48, pp. 15996-6011, (2015) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.