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Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed

ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Catalog No. ABIN101955
  • Target See all IgG products
    IgG
    Binding Specificity
    • 2382
    • 512
    • 424
    • 207
    • 132
    • 129
    • 95
    • 29
    • 28
    • 28
    • 22
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    Heavy & Light Chain
    Reactivity
    • 724
    • 699
    • 601
    • 477
    • 403
    • 243
    • 212
    • 176
    • 147
    • 138
    • 113
    • 112
    • 107
    • 102
    • 82
    • 59
    • 47
    • 28
    • 28
    • 16
    • 12
    • 10
    • 8
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Rabbit
    Host
    • 1778
    • 1576
    • 571
    • 241
    • 112
    • 111
    • 33
    • 31
    • 21
    • 19
    • 15
    • 11
    • 11
    • 1
    Donkey
    Clonality
    • 3434
    • 175
    • 1
    Polyclonal
    Conjugate
    • 588
    • 417
    • 415
    • 381
    • 282
    • 276
    • 244
    • 118
    • 108
    • 67
    • 66
    • 64
    • 61
    • 58
    • 52
    • 51
    • 48
    • 44
    • 44
    • 44
    • 41
    • 39
    • 35
    • 32
    • 31
    • 31
    • 30
    • 29
    • 29
    • 29
    • 29
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 27
    • 26
    • 26
    • 25
    • 22
    • 21
    • 19
    • 17
    • 17
    • 16
    HRP
    Application
    • 1952
    • 1838
    • 1806
    • 1519
    • 934
    • 910
    • 652
    • 478
    • 380
    • 236
    • 236
    • 234
    • 105
    • 103
    • 86
    • 67
    • 52
    • 52
    • 31
    • 28
    • 25
    • 21
    • 18
    • 16
    • 11
    • 9
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)
    Specificity
    IgG (H&L)
    Characteristics
    Anti-Rabbit IgG peroxidase conjugated antibody generated in donkey detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Immunogen
    Anti-Rabbit IgG was produced by repeated immunization with rabbit whole IgG molecule in donkey.
    Isotype
    IgG
  • Application Notes
    Anti-Rabbit IgG peroxidase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Restore with deionized water (or equivalent)
    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Preservative
    Gentamicin sulfate
    Handling Advice
    Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
    Storage
    4 °C
  • Klaric, Thomas, Dottori, Leong, Koblar, Lewis: "A reduction in Npas4 expression results in delayed neural differentiation of mouse embryonic stem cells." in: Stem cell research & therapy, Vol. 5, Issue 3, pp. 64, (2015) (PubMed).

    Gawriluk, Ko, Hong, Christenson, Rucker: "Beclin-1 deficiency in the murine ovary results in the reduction of progesterone production to promote preterm labor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 40, pp. E4194-203, (2015) (PubMed).

    Tidjane, Hachem, Zaid, Merhi, Gaboury, Girolami, Couture: "A primary role for kinin B1 receptor in inflammation, organ damage, and lethal thrombosis in a rat model of septic shock in diabetes." in: European journal of inflammation, Vol. 13, Issue 1, pp. 40-52, (2015) (PubMed).

  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background
    Synonyms: Rabbit Antibody peroxidase conjugated, Dk-a-Rabbit peroxidase conjugated IgG, Rabbit proxidase conjugated Antibody in donkey, Rabbit Secondary peroxidase conjugated Antibody, peroxidase.
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