Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed
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- Target See all IgG products
- IgG
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Rabbit
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Host
- Donkey
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Clonality
- Polyclonal
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Conjugate
- HRP
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)
- Specificity
- IgG (H&L)
- Characteristics
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Anti-Rabbit IgG peroxidase conjugated antibody generated in donkey detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm - Purification
- Preadsorption: Solid phase absorption
- Immunogen
- Anti-Rabbit IgG was produced by repeated immunization with rabbit whole IgG molecule in donkey.
- Isotype
- IgG
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- Application Notes
- Anti-Rabbit IgG peroxidase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Preservative
- Gentamicin sulfate
- Handling Advice
- Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
- Storage
- 4 °C
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A reduction in Npas4 expression results in delayed neural differentiation of mouse embryonic stem cells." in: Stem cell research & therapy, Vol. 5, Issue 3, pp. 64, (2015) (PubMed).
: "Beclin-1 deficiency in the murine ovary results in the reduction of progesterone production to promote preterm labor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 40, pp. E4194-203, (2015) (PubMed).
: "A primary role for kinin B1 receptor in inflammation, organ damage, and lethal thrombosis in a rat model of septic shock in diabetes." in: European journal of inflammation, Vol. 13, Issue 1, pp. 40-52, (2015) (PubMed).
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A reduction in Npas4 expression results in delayed neural differentiation of mouse embryonic stem cells." in: Stem cell research & therapy, Vol. 5, Issue 3, pp. 64, (2015) (PubMed).
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Synonyms: Rabbit Antibody peroxidase conjugated, Dk-a-Rabbit peroxidase conjugated IgG, Rabbit proxidase conjugated Antibody in donkey, Rabbit Secondary peroxidase conjugated Antibody, peroxidase.
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