Goat anti-Mouse IgG (Fc Region) Antibody (TRITC) - Preadsorbed
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- Target See all IgG products
- IgG
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Binding Specificity
- Fc Region
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Reactivity
- Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- TRITC
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Application
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Specificity
- IgG F(c)
- Characteristics
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- Preadsorption: immunoaffinity chromatography using Mouse IgG coupled to agarose beads
- Immunogen
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Immunogen: Mouse IgG F(c) fragment
- Isotype
- IgG
- Labeling Ratio
- 3.9
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- Application Notes
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 2.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Product is photosensitive and should be protected from light.
- Storage
- RT,4 °C,-20 °C
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
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Synonyms: Goat Anti-Mouse IgG F(c) rhodamine Conjugated Antibody, Goat Anti-Mouse IgG Fc Antibody rhodamine Conjugation, Goat Anti-Mouse IgG Fc Fragment Antibody TRITC Conjugated
Background: Anti-Mouse IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
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