Goat anti-Mouse IgG (Fc Region) Antibody

Catalog No. ABIN101851

Product Details

Target: IgG

Binding Specificity: Fc Region

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgG F(c)

Characteristics: Concentration Definition: by Refractometry

Purification: This product was prepared from monospecific antiserum by delipidation and defibrination.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum.  No reaction was observed against Mouse IgG F(ab).

Immunogen:

Immunogen: Mouse IgG F(c) fragment

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 85 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Expiry Date: 12 months

References

Li, Wang, Li: "Kinesin family member 20B regulates tongue cancer progression by promoting cell proliferation." in: Molecular medicine reports, Vol. 19, Issue 3, pp. 2202-2210, (2019) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: goat anti-Mouse IgG F(c) fragment Antibody, goat anti-Mouse IgG Fc fragment Antibody, rabbit anti Mouse IgG Fc

Background: Anti-Mouse IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.