Sheep anti-Mouse IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN101802

Supplier Product No.: 610-603-002

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Sheep

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.: 610-603-002

Supplier: Rockland

Purpose: Mouse IgG (H&L) Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum, Mouse IgG and Mouse Serum.

Characteristics: Anti-Mouse IgG Fluorescein Antibody generated in sheep detects reactivity to Mouse IgG.

Purification: Sheep Anti-Mouse IgG[H&L] Peroxidase Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Anti-Mouse IgG [H&L] was produced by repeated immunization with Mouse IgG whole molecule in sheep.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Mouse IgG Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:100,000 - 1:200,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Meade, Latorre-Margalef, Stallknecht, Krammer: "Development of an influenza virus protein microarray to measure the humoral response to influenza virus infection in mallards." in: Emerging microbes & infections, Vol. 6, Issue 12, pp. e110, (2018) (PubMed).

Duehr, Wohlbold, Oestereich, Chromikova, Amanat, Rajendran, Gomez-Medina, Mena, tenOever, García-Sastre, Basler, Munoz-Fontela, Krammer: "Novel Cross-Reactive Monoclonal Antibodies against Ebolavirus Glycoproteins Show Protection in a Murine Challenge Model." in: Journal of virology, Vol. 91, Issue 16, (2017) (PubMed).

Leung, Cuddy, Wang, Rommens, Glogauer: "Sbds is required for Rac2-mediated monocyte migration and signaling downstream of RANK during osteoclastogenesis." in: Blood, Vol. 117, Issue 6, pp. 2044-53, (2011) (PubMed).

Leung, Wang, Cuddy, Sun, Magalhaes, Grynpas, Glogauer: "Filamin A regulates monocyte migration through Rho small GTPases during osteoclastogenesis." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 25, Issue 5, pp. 1077-91, (2010) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Anti-Mouse IgG whole molecule antibody generated in sheep detects specifically Mouse IgG heavy and light chain. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.