Rabbit anti-Mouse IgG (Heavy & Light Chain) Antibody - Preadsorbed

Catalog No. ABIN101785

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag)

Specificity: IgG (H&L)

Cross-Reactivity: Mouse (Murine)

Characteristics: Anti-Mouse antibody generated in rabbit detects specifically mouse IgG (H&L). This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Mouse IgG was produced by repeated immunization with mouse IgG whole molecule in rabbit

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: The rabbit anti-mouse IgG antibody ABIN101785 is suitable for use in ELISA, immunohistochemistry, and Western Blot, CUT&RUN and CUT&Tag. Specific conditions for each assay should be optimized by the end user. General ABIN101785 dilution recommendations for different applications are as follows:

• ELISA: 1:20,000 - 1:40,000
• WB: 1:2,000 - 1:10,000
• IHC: 1:1,000 - 1:5,000
• CUT&RUN: 1:100
• CUT&Tag: 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

References

Fujiwara, Tanno, Sugishita, Kishi, Makino, Okada: "Preparation of optimized concanavalin A-conjugated Dynabeads® magnetic beads for CUT&Tag." in: PLoS ONE, Vol. 16, Issue 11, pp. e0259846, (2021) (PubMed).

Willcockson, Healton, Weiss, Bartholdy, Botbol, Mishra, Sidhwani, Wilson, Pinto, Maron, Skalina, Toro, Zhao, Lee, Hou, Yusufova, Meydan, Osunsade, David, Cesarman, Melnick, Sidoli, Garcia, Edelmann et al.: "H1 histones control the epigenetic landscape by local chromatin compaction. ..." in: Nature, (2020) (PubMed).

Vonk, van Dooremalen, Liv, Klumperman, Coffer, Saris, Lorenowicz: "Mesenchymal Stromal/stem Cell-derived Extracellular Vesicles Promote Human Cartilage Regeneration In Vitro." in: Theranostics, Vol. 8, Issue 4, pp. 906-920, (2019) (PubMed).

Kooijmans, Gitz-Francois, Schiffelers, Vader: "Recombinant phosphatidylserine-binding nanobodies for targeting of extracellular vesicles to tumor cells: a plug-and-play approach." in: Nanoscale, Vol. 10, Issue 5, pp. 2413-2426, (2019) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: rabbit anti-Mouse IgG Antibody, Rabbit-a-Mouse IgG (H&L), Mouse IgG Antibody in rabbit

Background: Anti-Mouse IgG antibody generated in rabbit detects specifically mouse IgG (H&L). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This anti-Mouse secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.