Rabbit anti-Mouse IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN101782

Supplier Product No.: 610-4302

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.: 610-4302

Supplier: Rockland

Purpose: Mouse IgG (H&L) Secondary Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.

Characteristics: Anti-Mouse IgG3 Fluorescein Antibody generated in rabbit detects reactivity to Mouse IgG3 (Gamma 3 chain).

Purification: HRP Secondary Antibody Conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.

Immunogen: Optional[Immunogen]: Mouse IgG whole molecule

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Mouse secondary antibody conjugated to horseradish peroxidase (HRP) generated in rabbit detects specifically Mouse IgG whole molecule. Anti-Mouse IgG peroxidase antibody has been tested by ELISA and western blot and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:10,000 - 1:30,000 ELISA Dilution: 1:50,000 - 1:300,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store mouse secondary antibody conjugate at 4° C prior to restoration.   For extended storage aliquot antibody and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References

Lim, Bae, Lee, Lee: "Palmitoyl‑RGD promotes the expression of dermal‑epidermal junction components in HaCaT cells." in: Molecular medicine reports, Vol. 26, Issue 4, (2022) (PubMed).

Amanat, Strohmeier, Rathnasinghe, Schotsaert, Coughlan, García-Sastre, Krammer: "Introduction of Two Prolines and Removal of the Polybasic Cleavage Site Lead to Higher Efficacy of a Recombinant Spike-Based SARS-CoV-2 Vaccine in the Mouse Model." in: mBio, Vol. 12, Issue 2, (2021) (PubMed).

Amanat, White, Miorin, Strohmeier, McMahon, Meade, Liu, Albrecht, Simon, Martinez-Sobrido, Moran, García-Sastre, Krammer: "An In Vitro Microneutralization Assay for SARS-CoV-2 Serology and Drug Screening." in: Current protocols in microbiology, Vol. 58, Issue 1, pp. e108, (2020) (PubMed).

Jiang, Moorthy, Patel, Kumar, Morgan, Alfonso, Huang, Lampidis, Isom, Barrientos, Fontanesi, Zhang: "Regulation of Mitochondrial Respiratory Chain Complex Levels, Organization, and Function by Arginyltransferase 1." in: Frontiers in cell and developmental biology, Vol. 8, pp. 603688, (2020) (PubMed).

Wakabayashi, Kono, Kozaka, Tahara, Kamiya, Goto: "Transcutaneous Codelivery of Tumor Antigen and Resiquimod in Solid-in-Oil Nanodispersions Promotes Antitumor Immunity." in: ACS biomaterials science & engineering, Vol. 5, Issue 5, pp. 2297-2306, (2019) (PubMed).

Kirkpatrick, Qiu, Wilson, Bahl, Krammer: "The influenza virus hemagglutinin head evolves faster than the stalk domain." in: Scientific reports, Vol. 8, Issue 1, pp. 10432, (2018) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secondary antibodies bind to the primary antibody to assist in detection, sorting and purification of target antigens. To enable detection, the secondary antibody must have specificity for the antibody species and isotype of the primary antibody being used and generally is conjugated. Rockland produces highly active antibodies and conjugates against mouse immunoglobulins. Anti-Mouse Secondary Antibodies are affinity-purified polyclonal antibodies with well-characterized specificity for mouse immunoglobulin classes, subclasses, and fragments. They are available as unlabeled antibodies as well as conjugates of alkaline phosphatase (AP), horseradish peroxidase (HRP), fluorescent conjugates, and biotin. This Anti-MOUSE IgG generated in rabbit is designed to detect heavy and light chains (H&L) and has been Peroxidase (HRP) conjugated. Rigorous quality control testing ensures that the finished product meets or exceeds out high standards for optimum performance in your assays. These secondary antibodies are used throughout various types of assays, including ELISA or Western Blot, Immunohistochemistry, Flow Cytometry. Optimal secondary antibody requires knowledge of the detection assay.