Rabbit anti-Mouse IgG (Heavy & Light Chain) Antibody

Catalog No. ABIN101776

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgG (H&L)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Purification: Anti-Mouse IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum.

Sterility: Sterile filtered

Immunogen:

Immunogen: Mouse IgG whole molecule

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Mouse IgG Secondary Antibodies are ideal for Western Blotting, Immunohistochemistry, ELISA as well as other antibody detection methods.

ELISA Dilution: 1:20,000 - 1:400,000

Western Blot Dilution: 1:2,000 - 1:10,000

Comment:

Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

References

Stamatiades, Tremblay, Bohm, Crozet, Bisht, Kao, Coelho, Fan, Yewdell, Davidson, Heeger, Diebold, Nimmerjahn, Geissmann: "Immune Monitoring of Trans-endothelial Transport by Kidney-Resident Macrophages." in: Cell, Vol. 166, Issue 4, pp. 991-1003, (2016) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: rabbit anti-Mouse IgG Antibody, rabbit anti-mouse IgG heavy and light chain antibody, rb anti-ms IgG heavy and light chain, Anti-Mouse Secondary Antibody

Background: Anti-Mouse IgG Antibody generated in rabbit detects reactivity to Mouse IgG heavy and light chains. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.