Rabbit anti-Mouse IgG (Heavy & Light Chain) Antibody

Catalog No. ABIN101774

Supplier Product No.: 110-4102

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.: 110-4102

Supplier: Rockland

Purpose: Mouse IgG (H&L) Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Mouse IgG and Mouse Serum.

Characteristics: Anti-Mouse Serum antibody detects mouse serum proteins. Serum proteins are those proteins remaining in portion of plasma after coagulation of blood, during which process the plasma protein fibrinogen is converted to fibrin and remains behind in the clot. Anti-Mouse Serum antibody is ideal for investigators involved in Cell Signaling, cellular biology and Signal Transduction research.

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Optional[Immunogen]: Mouse IgG whole molecule

Application Details

Application Notes: Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL

Concentration: 80 mg/mL

Buffer:

Buffer: None

Stabilizer: None

, Preservative:None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Kusch, Uecker, Liepold, Möbius, Hoffmann, Neumann, Werner, Jahn: "Partial Immunoblotting of 2D-Gels: A Novel Method to Identify Post-Translationally Modified Proteins Exemplified for the Myelin Acetylome." in: Proteomes, Vol. 5, Issue 1, (2017) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.