Donkey anti-Mouse IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN101709

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Donkey

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)

Specificity: IgG (H&L)

Characteristics: Anti-Mouse IgG whole molecule antibody generated in donkey detects specifically Mouse IgG whole molecule. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Immunogen: Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in donkey.

Isotype: IgG

Application Details

Application Notes: Suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other peroxidase antibody-based enzymatic assays requiring lot-to-lot consistency.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Gentamicin sulfate

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

References

Czulkies, Mastroianni, Lutz, Lang, Schwan, Schmidt, Lassmann, Zeiser, Aktories, Papatheodorou: "Loss of LSR affects epithelial barrier integrity and tumor xenograft growth of CaCo-2 cells." in: Oncotarget, Vol. 8, Issue 23, pp. 37009-37022, (2018) (PubMed).

Schorch, Heni, Zahaf, Brummer, Mione, Schmidt, Papatheodorou, Aktories: "Targeting oncogenic Ras by the Clostridium perfringens toxin TpeL." in: Oncotarget, Vol. 9, Issue 23, pp. 16489-16500, (2018) (PubMed).

Klaric, Thomas, Dottori, Leong, Koblar, Lewis: "A reduction in Npas4 expression results in delayed neural differentiation of mouse embryonic stem cells." in: Stem cell research & therapy, Vol. 5, Issue 3, pp. 64, (2015) (PubMed).

Hemmasi, Czulkies, Schorch, Veit, Aktories, Papatheodorou: "Interaction of the Clostridium difficile Binary Toxin CDT and Its Host Cell Receptor, Lipolysis-stimulated Lipoprotein Receptor (LSR)." in: The Journal of biological chemistry, Vol. 290, Issue 22, pp. 14031-44, (2015) (PubMed).

Litjens, Nguyen, Castro, Aromataris, Jones, Barritt, Rychkov: "Phospholipase C-gamma1 is required for the activation of store-operated Ca2+ channels in liver cells." in: The Biochemical journal, Vol. 405, Issue 2, pp. 269-76, (2007) (PubMed).

Dazy, Damiola, Parisey, Beug, Gandrillon: "The MEK-1/ERKs signalling pathway is differentially involved in the self-renewal of early and late avian erythroid progenitor cells." in: Oncogene, Vol. 22, Issue 58, pp. 9205-16, (2004) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Synonyms: Mouse Antibody, Gt-a-Mouse IgA, Mouse Antibody in Goat, Mouse Secondary Antibody