Donkey anti-Mouse IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN101697

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Donkey

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA

Specificity: IgG (H&L)

Characteristics: Anti-Mouse IgG (H&L) peroxidase conjugated antibody generated in donkey detects specifically mouse IgG (H&L). This secondary peroxidase conjugated antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Immunogen: Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in donkey

Isotype: IgG

Application Details

Application Notes: Antibody anti mouse peroxidase conjugated has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product. Antibody anti mouse peroxidase conjugated has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 2.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Gentamicin sulfate

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

References

Toots, Seppa, Jagomäe, Koppel, Pallase, Heinla, Terasmaa, Plaas, Vasar: "Preventive treatment with liraglutide protects against development of glucose intolerance in a rat model of Wolfram syndrome." in: Scientific reports, Vol. 8, Issue 1, pp. 10183, (2018) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Synonyms: Mouse IgG (H&L) Antibody, Dk-a-Mouse (H&L) Peroxidase conjugated, Mouse (H&L) Peroxidase Antibody in donkey, Donkey (H&L) peroxidase conjugated Secondary Antibody.