Goat anti-Monkey IgG Antibody (HRP)

Catalog No. ABIN101680

Supplier Product No.: 617-103-012

Product Details

Target: IgG

Reactivity: Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.: 617-103-012

Supplier: Rockland

Purpose: Monkey IgG gamma Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Monkey IgG and Monkey Serum.

Characteristics: Anti-Monkey IgM (mu chain) peroxidase conjugated antibody generated in goat detects specifically Monkey IgM (mu chain).

Purification: MONKEY IgG gamma Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Anti-Monkey IgG (gamma chain) was produced by repeated immunization with monkey IgG gamma heavy chain in goat.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Monkey IgG (gamma chain) peroxidase conjugated antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:1,000 - 1:85,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Luo, Liao, Zhang, Easterhoff, Wiehe, Gurley, Armand, Allen, Von Holle, Marshall, Whitesides, Pritchett, Foulger, Hernandez, Parks, Lloyd, Stolarchuk, Sawant, Peel, Yates, Dunford, Arora, Wang, Bowman et al.: "Tissue memory B cell repertoire analysis after ALVAC/AIDSVAX B/E gp120 immunization of rhesus macaques. ..." in: JCI insight, Vol. 1, Issue 20, pp. e88522, (2018) (PubMed).

Caro-Aguilar, Ottinger, Hepler, Nahas, Wu, Good, Batzloff, Joyce, Heinrichs, Skinner: "Immunogenicity in mice and non-human primates of the Group A Streptococcal J8 peptide vaccine candidate conjugated to CRM197." in: Human vaccines & immunotherapeutics, Vol. 9, Issue 3, pp. 488-96, (2015) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Anti-Monkey IgG (gamma chain) peroxidase conjugated antibody generated in goat detects specifically Monkey IgG (gamma chain). This secondary peroxidase conjugated antibody anti-Monkey is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.