Goat anti-Monkey IgG (Heavy Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed

Catalog No. ABIN101677

Product Details

Target: IgG

Binding Specificity: Heavy Chain

Reactivity: Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgG (gamma chain)

Characteristics: Anti-Monkey IgG alkaline phosphatase conjugated antibody generated in goat detects specifically Monkey IgG. This secondary alkaline phosphatase conjugated antibody anti-Monkey is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Sterility: Sterile filtered

Immunogen:

Immunogen: Monkey IgG gamma heavy chain

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-Monkey IgG alkaline phosphatase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunohistochemistry as well as other alkaline phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.

ELISA Dilution: 1:20,000

Western Blot Dilution: 1:500 - 1:2,500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Expiry Date: 12 months

References

Pollara, Jones, Huffman, Edwards, Dennis, Li, Jha, Goodman, Kumar, LaBranche, Montefiori, Fouda, Hope, Tomaras, Staats, Ferrari, Permar: "Bridging Vaccine-Induced HIV-1 Neutralizing and Effector Antibody Responses in Rabbit and Rhesus Macaque Animal Models." in: Journal of virology, Vol. 93, Issue 10, (2019) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: goat anti-Monkey IgG (gamma chain) alkaline phosphatase conjugated antibody, goat anti-Monkey IgG gamma antibody alk phos conjugation

Background: Anti-Monkey IgG alkaline phosphatase conjugated antibody generated in goat detects specifically Monkey IgG. This secondary alkaline phosphatase conjugated antibody anti-Monkey is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.