Rabbit anti-Human IgG (F(ab')2 Region) Antibody (HRP)

Catalog No. ABIN101631

Product Details

Target: IgG

Binding Specificity: F(ab')2 Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgG F(ab')2

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: Human IgG fragment

Isotype: IgG

Application Details

Application Notes: Anti-Human IgG F(ab') 2 antibody has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:8,500 to 1:72,000 of the reconstitution concentration is suggested for this product. This product is also suitable for Western Blot and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Gentamicin sulfate

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody