Rabbit anti-Human IgG (F(ab')2 Region) Antibody

Catalog No. ABIN101627

Supplier Product No.: 609-4118

Product Details

Target: IgG

Binding Specificity: F(ab')2 Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Supplier Product No.: 609-4118

Supplier: Rockland

Purpose: Human IgG F(ab')2 Antibody pre-absorbed

Cross-Reactivity (Details): Minimal crossreactivity against MOUSE Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, Human IgG F(ab')2 and Human Serum.  No reaction was observed against Human IgG F(c) or Mouse Serum Proteins.

Characteristics: Anti-Human IgG (H&L) generated in rabbit detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present.

Purification: Anti-Human IgG F(ab')2 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Anti-Human IgG F(ab')2 fragment was produced in rabbit by repeated immunization with human IgG F(ab')2 fragment.

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Human IgG F(ab')2 antibody has been tested by ELISA and dot blot and is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:3,000 - 1:15,000 ELISA Dilution: 1:350,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

References

Kameoka, Kishi, Koura, Yamakawa, Nagasawa, Ito, Matsuda, Suzuki, Nakayama, Suzuki: "Efficacy of a recombinant single-chain fragment variable region, VasSF, as a new drug for vasculitis." in: Drug design, development and therapy, Vol. 13, pp. 555-568, (2019) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Anti-Human IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind human IgG F(c) will not bind human IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG F(ab')2 Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.