Rabbit anti-Human IgG (F(ab')2 Region) Antibody - Preadsorbed

Catalog No. ABIN101618

Product Details

Target: IgG

Binding Specificity: F(ab')2 Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgG F(ab')2

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: immunoaffinity chromatography using Human antigens coupled to agarose beads

Sterility: Sterile filtered

Immunogen:

Immunogen: Human IgG F(ab')2 fragment

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Human IgG F(ab')2 antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

ELISA Dilution: 1:25,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 6.03 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Rabbit Anti Human IgG Fab2 Antibody, Rabbit Anti-Human IgG F(ab')2 Antibody, Rabbit Anti Human IgG Fab2 Fragment Antibody

Background: Anti-Human IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind human IgG F(c) will not bind human IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.