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Rabbit anti-Human IgG (F(ab')2 Region) Antibody

Rockland 609-4104 ELISA, IHC, WB Polyclonal IgG unconjugated
Rockland
Catalog No. ABIN101618
Supplier Product No.: 609-4104
  • Target See all IgG products
    IgG
    Binding Specificity
    • 2403
    • 511
    • 416
    • 148
    • 129
    • 128
    • 67
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 1
    • 1
    F(ab')2 Region
    Reactivity
    • 719
    • 692
    • 594
    • 465
    • 393
    • 240
    • 208
    • 176
    • 145
    • 137
    • 112
    • 106
    • 105
    • 101
    • 82
    • 58
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 8
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 1761
    • 1548
    • 569
    • 238
    • 112
    • 111
    • 32
    • 31
    • 21
    • 18
    • 15
    • 11
    • 11
    Rabbit
    Clonality
    • 3389
    • 175
    • 1
    Polyclonal
    Application
    • 2032
    • 1981
    • 1835
    • 1784
    • 983
    • 920
    • 896
    • 486
    • 420
    • 234
    • 234
    • 151
    • 99
    • 84
    • 65
    • 52
    • 51
    • 30
    • 28
    • 25
    • 20
    • 15
    • 11
    • 9
    • 8
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Supplier Product No.
    609-4104
    Supplier
    Rockland
    Purpose
    Human IgG F(ab')2 Antibody
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, Human IgG F(ab')2 and Human Serum.  No reaction was observed against Human IgG F(c).
    Characteristics
    Anti-Human IgG F(c) generated in rabbit detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .
    Purification
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads.
    Sterility
    Sterile filtered
    Immunogen
    Optional[Immunogen]: Human IgG F(ab')2 fragment
    Isotype
    IgG
  • Application Notes
    Application Note: Anti-Human IgG F(ab')2 antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:25,000 Other: User Optimized
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    6.03 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    , Preservative:0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background
    Anti-Human IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind human IgG F(c) will not bind human IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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