Rabbit anti-Human IgG (Heavy & Light Chain) Antibody (HRP)
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- Target See all IgG products
- IgG
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- HRP
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)
- Specificity
- IgG (H&L)
- Characteristics
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Anti-Human IgG whole molecule antibody generated in rabbit detects specifically human IgG whole molecule. This secondary antibody anti-Human is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm - Immunogen
- Anti-Human IgG whole molecule was produced by repeated immunization with Human IgG whole molecule in rabbit.
- Isotype
- IgG
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- Application Notes
- Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Restore with deionized water (or equivalent)
- Concentration
- 2.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Preservative
- Gentamicin sulfate
- Handling Advice
- Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
- Storage
- 4 °C
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Synonyms: Human Antibody, Rb-a-Human IgG, Human Antibody in Rabbit, Human Secondary Antibody
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