Goat anti-Human IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))
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- Target See all IgG products
- IgG
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Alkaline Phosphatase (AP)
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
- Supplier Product No.
- 609-1502
- Supplier
- Rockland
- Purpose
- Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Human IgG and Human Serum.
- Characteristics
- Anti-Human IgG (H&L) Peroxidase generated in goat detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present.
- Purification
- Anti-Human IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads.
- Sterility
- Sterile filtered
- Immunogen
- Optional[Immunogen]: Human IgG whole molecule
- Isotype
- IgG
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- Application Notes
- Application Note: Anti-Human IgG (H&L) Alkaline Phosphatase Antibody has been tested by ELISA, dot blot, and western blot and is suitable for Immunohistochemistry applications. Antibody should be optimized by end user for specific reactive conditions. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:4,500 Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.1 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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