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Goat anti-Goat IgG (Fc Region) Antibody - Preadsorbed

ELISA, IHC, WB Polyclonal IgG unconjugated
Catalog No. ABIN101444
  • Target See all IgG products
    IgG
    Binding Specificity
    • 2383
    • 511
    • 424
    • 207
    • 132
    • 129
    • 95
    • 29
    • 28
    • 28
    • 22
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    Fc Region
    Reactivity
    • 724
    • 699
    • 602
    • 477
    • 402
    • 243
    • 212
    • 176
    • 147
    • 138
    • 112
    • 112
    • 107
    • 102
    • 82
    • 59
    • 47
    • 28
    • 28
    • 16
    • 12
    • 10
    • 8
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Goat, Horse
    Host
    • 1777
    • 1576
    • 572
    • 241
    • 112
    • 111
    • 33
    • 31
    • 21
    • 19
    • 15
    • 11
    • 11
    • 1
    Goat
    Clonality
    • 3434
    • 175
    • 1
    Polyclonal
    Application
    • 1952
    • 1838
    • 1806
    • 1519
    • 934
    • 910
    • 652
    • 478
    • 380
    • 236
    • 236
    • 234
    • 106
    • 103
    • 86
    • 67
    • 53
    • 52
    • 31
    • 28
    • 25
    • 21
    • 18
    • 16
    • 11
    • 9
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    IgG F(c)
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: immunoaffinity chromatography using Horse IgG coupled to agarose beads
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Horse IgG F(c) fragment

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-Horse IgG F(c) antibody is suitable for ELISA, Western Blot and Immunohistochemistry applications. Antibody should be optimized by end user for specific reactive conditions.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    2.1 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background

    Synonyms: goat Anti-Horse IgG F(c) Antibody, goat Anti-Horse IgG Fc Fragment Antibody

    Background: Horse IgG F(c) antibody generated in goat recognizes the F(c) portion of horse IgG. It is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of horse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Anti-Horse IgG F(c) antibody is ideal for investigators involved in serum component protein research.

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