Goat anti-Armenian Hamster IgG (Heavy & Light Chain) Antibody

Catalog No. ABIN101378

Supplier Product No.: 619-101-002

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Armenian Hamster

Host: Goat

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Supplier Product No.: 619-101-002

Supplier: Rockland

Purpose: Armenian Hamster IgG (H&L) Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Armenian Hamster IgG and Armenian Hamster Serum.  Reactivity with Golden Syrian Hamster IgG is greatly reduced.

Characteristics: Anti-Armenian Hamster IgG Rhodamine Antibody generated in goat detects Armenian Hamster IgG.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Armenian Hamster IgG whole molecule

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Armenian Hamster IgG Antibody has been tested by ELISA and is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 ChIP Dilution: User Optimized Western Blot Dilution: 1:2,000 - 1:10,000 Immunoprecipitation Dilution: User Optimized ELISA Dilution: 1:20,000 - 1:40,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 5.03 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

References

Köchl, Thelen, Vanes, Brazão, Fountain, Xie, Huang, Lyck, Stein, Tybulewicz: "WNK1 kinase balances T cell adhesion versus migration in vivo." in: Nature immunology, Vol. 17, Issue 9, pp. 1075-83, (2017) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.