Goat anti-Guinea Pig IgG (Fc Region) Antibody - Preadsorbed

Catalog No. ABIN101326

Product Details

Target: IgG

Binding Specificity: Fc Region

Reactivity: Guinea Pig

Host: Goat

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgG F(c)

Characteristics: Anti-Guinea Pig IgG F(c) antibody generated in Goat detects specifically Guinea Pig IgG F(c). Anti-Guinea Pig IgG F(c) secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Guinea Pig IgG F(c) antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Sterility: Sterile filtered

Immunogen:

Immunogen: Guinea Pig IgG F(c) fragment

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Guinea Pig IgG F(c) antibody has been assayed against 1.0 μg of Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for Anti-guinea Pig IgG F(c) Antibody.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Guinea Pig IgG F(c) Antibody, Goat-a-Guinea Pig F(c), Guinea Pig F(c) in Goat, Goat anti-guinea pig F(c) Secondary Antibody.

Background: Anti-Guinea Pig IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of Guinea Pig IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Anti-Guinea Pig IgG F(c) antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.