Rabbit anti-Guinea Pig IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN101300

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA

Specificity: IgG (H&L)

Characteristics: Anti-Guinea Pig IgG (H&L) peroxidase conjugated antibody generated in rabbit detects specifically guinea pig IgG (H&L). This secondary peroxidase conjugated antibody anti-Guinea Pig is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Immunogen: Anti-Guinea Pig IgG was produced by repeated immunization with guinea pig IgG whole molecule in rabbit.

Isotype: IgG

Application Details

Application Notes: Antibody Anti-Guinea Pig IgG (H&L) peroxidase conjugated has been assayed against 1.0 µg of Guinea Pig IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Guinea Pig IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 2 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Gentamicin sulfate

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Synonyms: Guinea Pig IgG (H&L) Antibody, Rb-a-Guinea Pig IgG(H&L) Peroxidase conjugated, Guinea Pig IgG(H&L) Peroxidase Antibody in rabbit, Rabbit IgG (H&L) peroxidase conjugated Secondary Antibody.