Rabbit anti-Goat IgG (Fc Region) Antibody (HRP)

Catalog No. ABIN101255

Product Details

Target: IgG

Binding Specificity: Fc Region

Reactivity: Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA

Specificity: IgG F(c)

Characteristics: Anti-Goat IgG F(c) peroxidase conjugated antibody generated in rabbit detects specifically goat IgG F(c). This secondary peroxidase conjugated antibody anti-Goat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Immunogen: Anti-Goat IgG was produced by repeated immunization with Goat IgG fragment in rabbit.

Isotype: IgG

Application Details

Application Notes: Goat IgG F(c) peroxidase conjugated antibody has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 is suggested for this product. Anti-Goat IgG F(c) peroxidase conjugated antibody has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 is suggested for this product.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 2.0 mg/mL

Buffer: 0.01 M Potassium Phosphate, 0.14 M Sodium Chloride, pH 7.4
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA), IgG and Protease free Preservative: 0.01 % (w/v) Gentamicin Sulfate

Preservative: Gentamicin sulfate

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

References

Ma, Yan, Huang, Zhao, Zhu: "Downregulation of human epidermal growth factor receptor 2 by short hairpin RNA increases chemosensitivity of human ovarian cancer cells." in: Oncology letters, Vol. 9, Issue 5, pp. 2211-2217, (2015) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Synonyms: Goat IgG F(c) Antibody, Rb-a-Goat IgG F(c) Peroxidase conjugated, goat IgG F(c) Peroxidase Antibody in rabbit, Rabbit peroxidase conjugated Secondary Antibody.