Rabbit anti-Goat IgG (F(ab')2 Region) Antibody (FITC) - Preadsorbed
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- Target See all IgG products
- IgG
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Binding Specificity
- F(ab')2 Region
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Reactivity
- Goat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- FITC
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Application
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Specificity
- IgG F(ab')2
- Characteristics
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Goat IgG F(ab')2 fragment
- Isotype
- IgG
- Labeling Ratio
- 3.0
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- Application Notes
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- Comment
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Excitation/Emission wavelength: 494 nm/514 nm
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 2.0 mg/mL
- Buffer
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Product is photosensitive and should be protected from light.
- Storage
- RT,4 °C,-20 °C
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
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Synonyms: rabbit anti-goat IgG F(ab')2 FITC conjugated, rabbit anti-goat IgG F(ab')2 Fluorescein conjugated, rabbit anti-goat IgG Fab2 FITC conjugated, rabbit anti-goat IgG Fab2 Fluorescein conjugated
Background: Anti-Goat IgG F(ab')2 Fluorescein Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind goat IgG F(c) will not bind goat IgG F(ab')2 Molecules.
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