Rabbit anti-Goat IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN101212

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Purpose: Goat IgG (H&L) Secondary Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum.

Characteristics: Anti-Goat IgG F(ab')2 fluorescein Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind goat IgG F(c) will not bind goat IgG F(ab')2 Molecules.

Purification: Rabbit Anti-Goat IgG secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Goat IgG whole molecule

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Goat IgG Peroxidase conjugate has been tested by ELISA and western blot. This antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:5,000 - 1:20,000 ELISA Dilution: 1:50,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Secondary Antibody at 4° C prior to restoration.   For extended storage aliquot antibody and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References

Fan, Nørgaard, Bindesbøll, Lucas, Dalen, Babaie, Itkonen, Matthews, Nebb, Grønning-Wang: "LXRα Regulates Hepatic ChREBPα Activity and Lipogenesis upon Glucose, but Not Fructose Feeding in Mice." in: Nutrients, Vol. 9, Issue 7, (2018) (PubMed).

Gill, Cohen, Ayala-Figueroa, Forman-Rubinsky, Poggioli, Bickard, Parry, Pu, Hall, Sundaram: "Integrity of Narrow Epithelial Tubes in the C. elegans Excretory System Requires a Transient Luminal Matrix." in: PLoS genetics, Vol. 12, Issue 8, pp. e1006205, (2017) (PubMed).

Klaric, Thomas, Dottori, Leong, Koblar, Lewis: "A reduction in Npas4 expression results in delayed neural differentiation of mouse embryonic stem cells." in: Stem cell research & therapy, Vol. 5, Issue 3, pp. 64, (2015) (PubMed).

Bindesbøll, Fan, Nørgaard, MacPherson, Ruan, Wu, Pedersen, Steffensen, Yang, Matthews, Mandrup, Nebb, Grønning-Wang: "Liver X receptor regulates hepatic nuclear O-GlcNAc signaling and carbohydrate responsive element-binding protein activity." in: Journal of lipid research, Vol. 56, Issue 4, pp. 771-85, (2015) (PubMed).

Bischof, Randoll, Süßner, Henne-Bruns, Pinna, Knippschild: "CK1δ kinase activity is modulated by Chk1-mediated phosphorylation." in: PloS one, Vol. 8, Issue 7, pp. e68803, (2014) (PubMed).

Ronsyn, Daans, Spaepen, Chatterjee, Vermeulen, DHaese, Van Tendeloo, Van Marck, Ysebaert, Berneman, Jorens, Ponsaerts: "Plasmid-based genetic modification of human bone marrow-derived stromal cells: analysis of cell survival and transgene expression after transplantation in rat spinal cord." in: BMC biotechnology, Vol. 7, pp. 90, (2008) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.