Donkey anti-Goat IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed

Catalog No. ABIN101187

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Goat

Host: Donkey

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB)

Specificity: IgG (H&L)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Immunogen: Anti-Goat IgG was produced by repeated immunization with Goat IgG whole molecule in donkey.

Isotype: IgG

Application Details

Application Notes: Anti-Goat IgG (H&L) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Gentamicin sulfate

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

References

Oud, Tuijnenburg, Hempel, van Vlies, Ren, Ferdinandusse, Jansen, Santer, Johannsen, Bacchelli, Alders, Li, Davies, Dupuis, Cale, Wanders, Pals, Ocaka, James, Müller, Lehmberg, Strom, Engels, Williams et al.: "Mutations in EXTL3 Cause Neuro-immuno-skeletal Dysplasia Syndrome. ..." in: American journal of human genetics, Vol. 100, Issue 2, pp. 281-296, (2017) (PubMed).

Ellison, Gearheart, Porter, Ambruso: "IFN-γ alters the expression of diverse immunity related genes in a cell culture model designed to represent maturing neutrophils." in: PLoS ONE, Vol. 12, Issue 10, pp. e0185956, (2017) (PubMed).

Miller, Finnie, Bowden, Scholz, Oh, Kok, Burrell, Trinidad, Boyle, Li: "Preclinical efficacy studies of influenza A haemagglutinin precursor loop peptides as a potential vaccine." in: The Journal of general virology, (2011) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Synonyms: Goat Antibody, Dk-a-Goat IgG, Goat Antibody in Donkey, Goat Secondary Antibody