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Rabbit anti-Donkey IgG (Heavy & Light Chain) Antibody (Biotin)

Rockland 616-4602 ELISA, IHC, WB Polyclonal IgG Biotin
Rockland
Catalog No. ABIN101137
Supplier Product No.: 616-4602
  • Target See all IgG products
    IgG
    Binding Specificity
    • 2402
    • 511
    • 417
    • 148
    • 129
    • 128
    • 67
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 1
    • 1
    Heavy & Light Chain
    Reactivity
    • 720
    • 692
    • 594
    • 465
    • 393
    • 240
    • 208
    • 176
    • 145
    • 137
    • 112
    • 106
    • 105
    • 101
    • 82
    • 57
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 8
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Donkey
    Host
    • 1761
    • 1548
    • 569
    • 238
    • 112
    • 111
    • 32
    • 31
    • 21
    • 18
    • 15
    • 11
    • 11
    Rabbit
    Clonality
    • 3389
    • 175
    • 1
    Polyclonal
    Conjugate
    • 583
    • 418
    • 414
    • 377
    • 274
    • 262
    • 235
    • 119
    • 106
    • 73
    • 67
    • 64
    • 61
    • 58
    • 58
    • 50
    • 44
    • 43
    • 40
    • 39
    • 39
    • 37
    • 35
    • 32
    • 32
    • 31
    • 30
    • 29
    • 29
    • 29
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 27
    • 26
    • 26
    • 25
    • 22
    • 21
    • 19
    • 17
    • 17
    • 16
    Biotin
    Application
    • 2032
    • 1981
    • 1835
    • 1784
    • 983
    • 920
    • 896
    • 486
    • 420
    • 234
    • 234
    • 151
    • 99
    • 84
    • 65
    • 52
    • 51
    • 30
    • 28
    • 25
    • 20
    • 15
    • 11
    • 9
    • 8
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Supplier Product No.
    616-4602
    Supplier
    Rockland
    Purpose
    Donkey IgG (H&L) Antibody Biotin Conjugated
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Donkey IgG and Donkey Serum.
    Characteristics
    Anti-Donkey IgG Alkaline Phosphatase Antibody generated in rabbit detects donkey IgG.
    Purification
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    Immunogen
    Optional[Immunogen]: Donkey IgG whole molecule
    Isotype
    IgG
  • Application Notes
    Application Note: This product has been assayed against 1.0 μg of Donkey IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:45,000 to 1:150,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
    Concentration
    2.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background
    Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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